| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CELLA, Emelia | SO | Affton High School | 1:02.17 | Verified |
| 2 | HANSON, Sarah | JR | Lutheran South High School | 59.74 | Verified |
| 3 | GOMILLER, Edith | SO | Lift for Life Academy Charter Hi | 58.07 | Verified |
| 4 | LADOUCEUR, Gracianaelle | SO | Bayless High School | 56.49 | Verified |
| 5 | GARCIA-PINTOR, Joy | SO | Gateway Science Charter | 57.20 | Verified |
| 6 | SCARBROUGH, Makyah | JR | Bayless High School | 58.50 | Verified |
| 7 | TRODUS, Lauren | FR | Clayton High School | 1:00.21 | Verified |
| 8 | SHAFFER, Michelle | SO | Affton High School | 1:03.16 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CONNERS, Jaidyn | FR | University City High School | 55.51 | Verified |
| 2 | NASSAH, Goka | FR | Lift for Life Academy Charter Hi | 53.38 | Verified |
| 3 | WATSON, Haily | SR | Lutheran South High School | 53.23 | Verified |
| 4 | DIXON, Laila | JR | University City High School | 51.84 | Verified |
| 5 | MOORE, Gabrielle | SO | John Burroughs High School | 52.37 | Verified |
| 6 | MOORE, Madison | SO | John Burroughs High School | 53.37 | Unverified: |
| 7 | CROSS, Cesarine | SO | Clayton High School | 54.78 | Verified |
| 8 | RICHARDSON, Mariah | FR | STEAM Academy at McCluer South-B | 56.38 | Unverified: 11th Annual Bob G. Brunette Invi |