| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FLOOD, Nicholas | JR | Whitfield High School | NT | Unverified: |
| 4 | VAHEY, Camden | FR | Vianney High School | 1:07.12 | Verified |
| 5 | OSTLER, Amos | SO | Principia High School | NT | Unverified: |
| 6 | JONES, Javion | FR | Gateway High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VILLARREAL, Braulio | FR | Gateway Science Charter | 1:03.09 | Verified |
| 2 | NANGLE, Justin | SO | Priory High School | 59.43 | Unverified: |
| 3 | LUMPKIN, Ormani | FR | Lift for Life Academy Charter Hi | 59.16 | Verified |
| 4 | SCHMEROLD, Jacob | SO | Priory High School | 58.74 | Verified |
| 5 | SIMS, Dennis | SO | University City High School | 58.93 | Verified |
| 6 | MILLER, Jaylen | JR | Bayless High School | 59.32 | Verified |
| 7 | LINDSEY, Jacob | SO | Vianney High School | 1:00.98 | Verified |
| 8 | SUN, Samuel | JR | Clayton High School | 1:04.52 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOODS, Connor | JR | John Burroughs High School | 57.77 | Verified |
| 2 | HUYETTE, Logan | SO | Clayton High School | 57.46 | Verified |
| 3 | RILEY, Corbin | FR | John Burroughs High School | 56.23 | Verified |
| 4 | TAYLOR, Tim | SR | Whitfield High School | 55.62 | Verified |
| 5 | LANDERS, Bryan | SO | Lift for Life Academy Charter Hi | 55.67 | Verified |
| 6 | SHELTON, Trent | JR | Gateway High School | 56.54 | Verified |
| 7 | WATT HOVEN, Zane Oliver | JR | University City High School | 57.66 | Verified |
| 8 | KINSER, Alexander | SO | Lutheran South High School | 58.70 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JELTES, Aeden | JR | Gateway Science Charter | 54.86 | Verified |
| 2 | LANE, Jesse | SO | Brentwood High School | 54.34 | Verified |
| 3 | WARRICK, Luke | SR | Principia High School | 52.81 | Verified |
| 4 | CARTER, Jordan | JR | Lutheran North High School | 52.31 | Verified |
| 5 | LEONARD, David | JR | St. Mary's (St. Louis) High Scho | 52.76 | Unverified: |
| 6 | HAWKINS, Logan | SO | Brentwood High School | 53.14 | Verified |
| 7 | HICKERSON, Brenton | JR | Lutheran South High School | 54.68 | Verified |
| 8 | PASHKOV, Christopher | SR | Affton High School | 55.22 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | UEBARI, David | SR | Bayless High School | 51.66 | Verified |
| 2 | ROHAN, Henry | FR | MICDS | 51.56 | Unverified: |
| 3 | WILLIAMS, Tristan | JR | MICDS | 50.39 | Verified |
| 4 | MCINTOSH, Donovan | SR | St. Mary's (St. Louis) High Scho | 49.24 | Verified |
| 5 | CLARK, Michael | FR | Lutheran North High School | 49.37 | Verified |
| 6 | JOHNSON, Larry | SR | Hazelwood Central High School | 50.40 | Verified |
| 7 | LOVELACE, Josiah | JR | Affton High School | 51.61 | Verified |
| 8 | ANTHONY, Deontae | SO | Hazelwood Central High School | 52.29 | Verified |