| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STROUD, Payton | JR | University City High School | NT | Unverified: |
| 2 | JOHNSON, Catherine | SO | Gateway Science Charter | 1:23.22 | Verified |
| 3 | WOLPERT, Claire | FR | Clayton High School | 1:13.72 | Verified |
| 4 | WASSERMAN, Jenna | FR | Whitfield High School | 1:10.21 | Verified |
| 5 | SIMON, LaTandy | SO | University City High School | 1:12.15 | Verified |
| 6 | BAUM, Eleanor | JR | Clayton High School | 1:14.12 | Verified |
| 7 | CAROTHERS, Kennedy | JR | Gateway High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Gabrielle | SO | John Burroughs High School | 1:08.95 | Verified |
| 2 | GARCIA-PINTOR, Joy | SO | Gateway Science Charter | 1:07.34 | Verified |
| 3 | WORTHEM, Ayanna | FR | Lift for Life Academy Charter Hi | 1:04.84 | Verified |
| 4 | CROMER, Ja'Kaylah | JR | STEAM Academy at McCluer South-B | 1:03.43 | Unverified: |
| 5 | KASPAREK, Ella | SR | Lutheran South High School | 1:04.09 | Verified |
| 6 | GREEN, Cheyenne | JR | STEAM Academy at McCluer South-B | 1:05.18 | Unverified: |
| 7 | COUSINS, Addison | FR | Affton High School | 1:08.37 | Verified |
| 8 | WILLIAMS, Alaya | FR | Lift for Life Academy Charter Hi | 1:09.72 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAUGHTRY, Anna | SO | Affton High School | 1:02.52 | Verified |
| 2 | DOW, Lilly | SR | Principia High School | 1:01.68 | Verified |
| 3 | GARNER, Genesis | FR | Lutheran North High School | 1:01.13 | Verified |
| 4 | SAITZ, Elizabeth | SR | Lutheran South High School | 1:00.18 | Verified |
| 5 | WARD, Breaunna | SO | John Burroughs High School | 1:00.49 | Verified |
| 6 | GURLEY, Derriyah | FR | Bayless High School | 1:01.50 | Verified |
| 7 | STRINGFELLOW, Eryana | JR | Lutheran North High School | 1:02.02 | Verified |
| 8 | BROWN, Tiara | JR | Gateway High School | 1:02.77 | Verified |