| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 5 | Affton High School | A | Davis | Fuller | Allen | Moehlenbrock | NT | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Gateway High School | A | Clay | Moore | Warren | Calvin | NT | Verified |
| 2 | John Burroughs High School | A | Goffstein | Foote | Dharmarajan | Campbell | 46.37 | Verified |
| 3 | University City High School | A | Holland | Nylon | Cooley | Jennings | 48.00 | Unverified: Tiger Town Invitational |
| 4 | Lutheran South High School | A | Samking | Woods | Hoehner | Grus | 45.55 | Verified |
| 5 | Brentwood High School | A | Clay | Hawkins | Lane | Phillips | 45.61 | Verified |
| 6 | Vianney High School | A | Garrett | Fronabarger | Pullam | Battle | 46.11 | Verified |
| 7 | Principia High School | A | Ejimadu | Dannull | Mugabe | Udoekong | 47.06 | Verified |
| 8 | Whitfield High School | A | Carter | Chien | Babusis | Goldstein | 50.97 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lift for Life Academy Charter Hi | A | Hightower | Jones | Ridgel | Young | 45.51 | Verified |
| 2 | St. Mary's (St. Louis) High Scho | A | Strong | Washington | Hendricks | Roberts | 44.15 | Verified |
| 3 | Clayton High School | A | Anderson-Jennings | Chatman | Handzic-Smith | Aningo | 45.66 | Verified |
| 4 | MICDS | A | Reddic | Clay | Lester | Lindsey | 42.98 | Verified |
| 5 | Hazelwood Central High School | A | Anthony | Atkins | Martin | Williams | NT | Verified |
| 6 | Lutheran North High School | A | Bass | Dodolewa | Jefferson | Van Hook | 43.67 | Verified |
| 7 | Bayless High School | A | Sestan | Uebari | Jones | Patton | 45.42 | Verified |
| 8 | Chaminade College Preparatory Sc | A | Collins | Jenkins | Kai) Robinson | Taylor | 45.52 | Verified |