| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MEDINA, Luciana | JR | Nerinx Hall High School | 2:22.66 | Verified |
| 2 | DIGUISEPPE, Macie | SO | St. Joseph's Academy | 2:24.45 | Verified |
| 3 | SINGER, Rachel | SR | Villa Duchesne High School | 2:27.85 | Verified |
| 4 | MCKENZIE, Mary | FR | Cor Jesu Academy | 2:30.22 | Verified |
| 5 | STANN, Kailyn | FR | Nerinx Hall High School | 2:35.82 | Verified |
| 6 | LUNA, Maria | JR | Cor Jesu Academy | 2:36.19 | Verified |
| 7 | GUENTHER, Cate | JR | St. Joseph's Academy | 2:36.53 | Verified |
| 8 | KAISER, Grace | SR | Nerinx Hall High School | 2:39.75 | Verified |
| 9 | LOCHT, Emma | SO | Visitation Academy | 2:43.52 | Verified |
| 10 | PROBST, Bridget | JR | Cor Jesu Academy | 2:47.09 | Verified |
| 11 | PFEIFFER, Eleanor | SO | Ursuline Academy | 3:00.61 | Verified |
| 12 | VANNOY, Ryan | SR | Ursuline Academy | 3:23.00 | Verified |
| 13 | KRAEMER, Heidi | JR | Visitation Academy | 3:30.81 | Verified |
| 14 | BISHARA, Lola | FR | Visitation Academy | 3:33.10 | Verified |