| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HUTCHINSON, Chloe | FR | Incarnate Word Academy | NT | Unverified: |
| 3 | RAMMACHER, Lilly | SR | Villa Duchesne High School | 1:03.66 | Verified |
| 4 | MCCLURE, Clare | FR | Nerinx Hall High School | 1:05.02 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GILJUM, Kaylie | JR | St. Joseph's Academy | 1:02.86 | Verified |
| 2 | CRUMP, Samira | FR | Incarnate Word Academy | 59.34 | Verified |
| 3 | ESKER, Lexi | SR | St. Joseph's Academy | 55.25 | Verified |
| 4 | MOORE, Camille | SR | Incarnate Word Academy | 57.22 | Verified |
| 5 | PADBERG, Molly | JR | Nerinx Hall High School | 59.52 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CERNY, Maria | SO | Cor Jesu Academy | 54.21 | Verified |
| 2 | RICHART, Claire | SR | Visitation Academy | 53.88 | Verified |
| 3 | ROLWES, Bridget | SR | Cor Jesu Academy | 50.17 | Verified |
| 4 | HANNEKEN, Madi | SR | St. Joseph's Academy | 50.41 | Verified |
| 5 | JACKNEWITZ, Analise | JR | Nerinx Hall High School | 54.01 | Verified |
| 6 | BOOCK, Sarah | JR | Cor Jesu Academy | 54.25 | Verified |