| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLYNE, Rosemary | FR | Nerinx Hall High School | 1:14.13 | Verified |
| 2 | FISCHER, Sydney | FR | Ursuline Academy | 1:10.81 | Verified |
| 3 | SMITH, Avery | SR | Visitation Academy | 1:08.73 | Verified |
| 4 | MEYERS, Samantha | FR | Visitation Academy | 1:10.76 | Verified |
| 5 | ANTISDEL, Adali | FR | Visitation Academy | 1:13.83 | Verified |
| 6 | MORIE, Eva | SR | Cor Jesu Academy | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KAUSHIK, Lydia | JR | St. Joseph's Academy | 1:08.23 | Verified |
| 2 | BORCHERS, Mary | FR | St. Joseph's Academy | 1:06.89 | Verified |
| 3 | BOGNAR, Ava | FR | Cor Jesu Academy | 1:06.18 | Verified |
| 4 | WEIS, Emme | SO | Villa Duchesne High School | 1:06.51 | Verified |
| 5 | HENNESSEY, Elianna | FR | Nerinx Hall High School | 1:07.50 | Unverified: |
| 6 | RODGERS, Aaliyah | SO | Incarnate Word Academy | 1:08.70 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TRAVERS, Mary | SR | St. Joseph's Academy | 1:05.23 | Unverified: Chaminade Quad |
| 2 | BRECKENRIDGE, Dylan | FR | Cor Jesu Academy | 1:03.72 | Verified |
| 3 | MEDINA, Luciana | JR | Nerinx Hall High School | 58.62 | Verified |
| 4 | FLOWERS, Chavi | SR | Incarnate Word Academy | 58.62 | Verified |
| 5 | BRIDGES, Destiny | SR | Incarnate Word Academy | 1:04.31 | Verified |
| 6 | BRYOWSKY, Niamh | FR | Ursuline Academy | 1:05.32 | Verified |