| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ursuline Academy | A | Teng | Teng | Fischer | Bryowsky | 58.53 | Verified |
| 3 | Visitation Academy | A | Lane-Liwo | Peoples | Kendall | Antisdel | 55.77 | Verified |
| 4 | Villa Duchesne High School | A | Irvin | Trevino | O'Daniel | Wilson | 58.04 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Joseph's Academy | A | Rybak | Edmiston | Borchers | Hendricks | 53.02 | Verified |
| 3 | Incarnate Word Academy | A | White | Flowers | Tobias | Allen | 48.84 | Verified |
| 4 | Cor Jesu Academy | A | Stoeckel | Jacoby | Cerny | Lorms | 52.69 | Verified |
| 5 | Nerinx Hall High School | A | Hughes | Gallagher | Johnson | McConnell | 54.54 | Verified |