| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LUNA, Adrienne | SR | Cor Jesu Academy | 5:14.69 | Verified |
| 2 | SINGER, Rachel | SR | Villa Duchesne High School | 5:27.14 | Verified |
| 3 | MCKENZIE, Mary | FR | Cor Jesu Academy | 5:28.58 | Verified |
| 4 | AMANN, Savannah | FR | St. Joseph's Academy | 5:28.81 | Verified |
| 5 | BADER, Jessica | SR | Visitation Academy | 5:31.03 | Verified |
| 6 | LUNA, Maria | JR | Cor Jesu Academy | 5:35.68 | Verified |
| 7 | STANN, Kailyn | FR | Nerinx Hall High School | 5:38.70 | Verified |
| 8 | JACKSON, Ella | FR | St. Joseph's Academy | 5:46.01 | Verified |
| 9 | KAISER, Grace | SR | Nerinx Hall High School | 5:52.48 | Verified |
| 10 | NEFF, Sadie | SO | St. Joseph's Academy | 6:08.83 | Verified |
| 11 | PFEIFFER, Eleanor | SO | Ursuline Academy | 6:40.53 | Verified |
| 12 | DREBES, Sarah Ann | SR | Nerinx Hall High School | 6:45.06 | Verified |
| 13 | REDD, Bella | JR | Villa Duchesne High School | 7:25.47 | Verified |
| 14 | KRAEMER, Heidi | JR | Visitation Academy | 7:44.98 | Verified |
| 15 | BISHARA, Lola | FR | Visitation Academy | NT | Unverified: |