| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ursuline Academy | A | Rathouz | Fischer | Whitlock | Teng | 2:09.39 | Verified |
| 3 | Visitation Academy | A | Lane-Liwo | Peoples | Smith | Kendall | 1:59.84 | Verified |
| 4 | Villa Duchesne High School | A | Irvin | Bussen | Rammacher | Weis | 2:04.66 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Incarnate Word Academy | A | Smart | White | Griffin | Gordon | 1:54.94 | Verified |
| 3 | Cor Jesu Academy | A | Stoeckel | Jacoby | Cerny | Lorms | 1:50.87 | Verified |
| 4 | St. Joseph's Academy | A | Bittle | Edmiston | Kirsch | Malone | DQ | Verified |
| 5 | Nerinx Hall High School | A | Hughes | Padberg | Albillo | McConnell | 1:57.45 | Verified |