| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Incarnate Word Academy | A | Beard | Spottsville | Rodgers | Jackson | DNS | Verified |
| 2 | Cor Jesu Academy | A | Figliola | Bognar | Morie | Breckenridge | 4:34.48 | Verified |
| 3 | St. Joseph's Academy | A | Amann | Borchers | Diguiseppe | Hanneken | 4:19.14 | Verified |
| 4 | Nerinx Hall High School | A | Jacknewitz | Medina | Kaiser | Stann | 4:24.07 | Verified |
| 5 | Visitation Academy | A | Smith | Antisdel | Richart | Kendall | NT | Verified |