| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Nerinx Hall High School | A | Jacknewitz | Stann | Kaiser | Medina | 9:58.67 | Verified |
| 2 | Cor Jesu Academy | A | Stirton | McKenzie | Luna | Luna | 10:00.26 | Verified |
| 3 | St. Joseph's Academy | A | Diguiseppe | Theiling | Travers | Amann | 10:13.64 | Verified |
| 4 | Visitation Academy | A | Locht | Richart | Boedefeld | Bader | 10:49.25 | Verified |