| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pittsfield | A | Motley | Hoover | Marable | Petty | 1:39.20 | Unverified: |
| 2 | Kirksville High School | A | Harvey | Elsea | Omehele | Bedford | 1:36.75 | Verified |
| 3 | Rushville (R.-Industry) | A | 1:36.30 | Verified | ||||
| 4 | Ft. Zumwalt South High School | A | Beck | Godefroid | Pilger | Bates | 1:36.61 | Verified |
| 5 | Mark Twain High School | A | Tobin | Zumwalt | Bennett | Cruz | 1:39.09 | Verified |
| 6 | Palmyra High School | A | Dames | Harper | Hicks | Redd | 1:39.86 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bowling Green High School | A | Jennings | Starks | Dalton | Bryant | 1:34.53 | Verified |
| 2 | Ft. Zumwalt North High School | A | Baker | Miller | Hamilton | Futrell | 1:33.14 | Verified |
| 3 | Hannibal High School | A | Essig | Turner | Bergheger | Williams | 1:30.75 | Verified |
| 5 | Jacksonville (H.S.) | A | Reed | Armstrong | Davis | Ackerman | 1:33.56 | Verified |
| 6 | Quincy (Sr.) | A | Homan | Stice | Moore | Brockmiller | 1:35.09 | Verified |