| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mark Twain High School | A | Boleach | Hudelson | Dotson | Ross | 1:58.80 | Verified |
| 3 | Ft. Zumwalt South High School | A | Moller | Hendrix | Krapf | Moore | 1:57.66 | Verified |
| 4 | Bowling Green High School | A | Deters | Charlton | Turner | Ortega | 1:58.00 | Unverified: |
| 5 | Jacksonville (H.S.) | A | Thompson | Davis | Flick | Adams-Mack | 2:01.47 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty | A | 1:55.71 | Verified | ||||
| 2 | Palmyra High School | A | McBride | Compton | Loman | White | 1:51.45 | Verified |
| 3 | Hannibal High School | A | Brown | Stewart | LaJoy | Dean | 1:48.49 | Verified |
| 4 | Quincy (Sr.) | A | Reinhardt | Barbagiovanni | Moore | Crider | 1:49.91 | Verified |
| 5 | Kirksville High School | A | Baumgartner | Butler | Harris | Thomas | 1:52.64 | Verified |
| 6 | Pittsfield | A | Waters | Wyatt | Patterson | Preist | 1:55.97 | Verified |