| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Carthage (Illini West) | A | Gilpin | Gille | Jones | Mudd | 3:48.10 | Unverified: |
| 2 | Jacksonville (H.S.) | A | Ackerman | Finn | Armstrong | Armstrong | 3:47.05 | Verified |
| 3 | Hannibal High School | B | Worthington | Thompson | Bailey | Pociask | 3:45.00 | Unverified: |
| 4 | Kirksville High School | A | Elsea | Bedford | Novencido | Harvey | 3:45.77 | Verified |
| 5 | Pittsfield | A | Oitker | Poor | Veile | Petty | 3:47.10 | Unverified: |
| 6 | Liberty | A | 4:07.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Preston | Black | Northcutt | McLeod | 3:37.67 | Verified |
| 2 | Hannibal High School | A | Essig | Sharkey | Turner | Nichols | 3:34.76 | Verified |
| 3 | Ft. Zumwalt North High School | A | Miller | Ostmann | Friend | Page | 3:32.25 | Verified |
| 4 | Rushville (R.-Industry) | A | 3:34.20 | Verified | ||||
| 5 | Ft. Zumwalt South High School | A | Walker | Bass | Bates | Godefroid | 3:35.38 | Verified |
| 6 | Quincy (Sr.) | A | Homan | Lithila | Altman | Brockmiller | 3:41.26 | Verified |