| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty | A | 4:45.26 | Verified | ||||
| 2 | Pittsfield | A | Waters | Patterson | Ten Eyck | Wyatt | 4:25.30 | Unverified: |
| 3 | Kirksville High School | A | Butler | Herbst | Thomas | Harris | 4:20.03 | Verified |
| 4 | Quincy (Sr.) | A | Alexander | Moore | Schuering | Venvertloh | 4:20.29 | Verified |
| 5 | Hannibal High School | A | Ball | Groth | Coniglio | Dorsey | 4:26.09 | Verified |
| 6 | Ft. Zumwalt South High School | A | Pawlik | Fenton | Ritter | Harper | NT | Verified |