| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MCMATH, Marianna | JR | Hannibal High School | NT | Unverified: |
| 3 | FRIEDEN, Madison | SO | Pittsfield | 33.40 | Verified |
| 4 | HENDRIX, Jada | SO | Ft. Zumwalt South High School | 35.73 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MOLLER, Kennedy | SO | Ft. Zumwalt South High School | 31.69 | Verified |
| 3 | GUNNING, Kennedy | FR | Carthage (Illini West) | 29.38 | Verified |
| 4 | SCHELL, Emma | FR | Liberty | 29.41 | Verified |
| 5 | JOHNSON, Nevaeh | SO | Hannibal High School | 31.20 | Verified |
| 6 | DAVIS, Kimmy | JR | Jacksonville (H.S.) | 32.06 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHARLTON, Kaylyn | JR | Bowling Green High School | 29.06 | Verified |
| 2 | CRANE, Kameil | JR | Hannibal High School | 28.40 | Verified |
| 3 | BARBAGIOVANNI, Alden | SR | Quincy (Sr.) | 28.25 | Verified |
| 4 | BRYAN, Josie | JR | Carthage (Illini West) | 28.34 | Verified |
| 5 | THOMAS, Maya | SR | Kirksville High School | 28.50 | Verified |
| 6 | DAVIS, McKenna | FR | Liberty | 29.11 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMPSON, Coriana | FR | Jacksonville (H.S.) | 28.00 | Verified |
| 2 | WHITE, Taytum | JR | Palmyra High School | 27.12 | Verified |
| 3 | VENVERTLOH, Anna | SO | Quincy (Sr.) | 26.69 | Verified |
| 4 | DEAN, Sameya | SO | Hannibal High School | 26.90 | Verified |
| 5 | BOLEACH, Madison | JR | Mark Twain High School | 27.46 | Verified |
| 6 | HARRIS, Mya | FR | Kirksville High School | 28.18 | Verified |