| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pittsfield | A | Bonds | Jackson | Poor | Graham | 46.62 | Verified |
| 3 | Kirksville High School | A | Mendez | Mukatu | Novencido | Omehele | NT | Verified |
| 4 | Ft. Zumwalt South High School | A | Beck | Butler | Pilger | Godefroid | 46.37 | Verified |
| 5 | Mark Twain High School | A | Tobin | Cruz | Misner | Bennett | NT | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rushville (R.-Industry) | A | 45.40 | Verified | ||||
| 2 | Ft. Zumwalt North High School | A | Jones | Miller | Henderson | Futrell | 44.23 | Verified |
| 3 | Hannibal High School | A | Whiteside | Turner | Bergheger | Williams | 43.72 | Verified |
| 4 | Jacksonville (H.S.) | A | Reed | Armstrong | Owens | Ron Mitchell | 44.10 | Verified |
| 6 | Quincy (Sr.) | A | Moore | Altman | Stice | Bruening | 45.76 | Verified |