| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Jacksonville (H.S.) | A | Thompson | Lovell | Davis | Adams-Mack | 56.97 | Verified |
| 3 | Ft. Zumwalt South High School | A | Moller | Reese | Krapf | Moore | 54.73 | Verified |
| 4 | Mark Twain High School | A | Boleach | Hudelson | Hawn | Ross | 55.24 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Liberty | A | NT | Verified | ||||
| 2 | Palmyra High School | A | Voss | Loman | McBride | White | 52.71 | Verified |
| 3 | Hannibal High School | A | Stewart | LaJoy | Dean | Hark | 51.13 | Verified |
| 4 | Quincy (Sr.) | A | Hodges | Crider | Moore | Alexander | 52.44 | Verified |
| 5 | Bowling Green High School | A | Dean | Berry | Johnson | Turner | 53.55 | Verified |