| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | DeSmet Jesuit High School | A | Hatcher | Byas | Griffin | Terry | 1:34.07 | Verified |
| 4 | St. Louis University High School | A | Harris | Oliphant | Bailey | Wingo | 1:28.20 | Verified |
| 5 | Christian Brothers College High | A | McClellan | Van | Tillman | Leicht | 1:31.77 | Verified |
| 6 | Vianney High School | A | Ivory | Pullam | Abeln | Selsor | 1:38.03 | Verified |