| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | Ivory | Turmelle | Abeln | Lindsey | 3:57.47 | Verified |
| 3 | Christian Brothers College High | A | Dubose | Hayden | Leicht | Wertel | 3:41.19 | Unverified: Jim Farrell Invitational |
| 4 | St. Louis University High School | A | Mayer | Lowe | Moore | Oliphant | 3:24.81 | Verified |
| 5 | Chaminade College Preparatory Sc | A | Cattoor | Hardeman | Piontek | Tim) Bailey | 3:40.17 | Verified |
| 6 | DeSmet Jesuit High School | A | Thomas | Byas | Terry | Griffin | 3:45.80 | Verified |