| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WERTEL, Dylan | SR | Christian Brothers College High | 48.29 | Verified |
| 4 | GARRETT, John | SO | Vianney High School | 45.78 | Verified |
| 5 | SUZUKI, Yoshitaka | SR | Chaminade College Preparatory Sc | 46.66 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PIONTEK, Samuel | SR | Chaminade College Preparatory Sc | 43.97 | Verified |
| 2 | MAYER, Steffan | SR | St. Louis University High School | 43.12 | Verified |
| 3 | SPAETE JR, Christopher | SR | Christian Brothers College High | 42.88 | Unverified: |
| 4 | OLIPHANT, Jacobi | JR | St. Louis University High School | 39.17 | Verified |
| 5 | HURLEY, Lucas | SO | Vianney High School | 39.97 | Verified |
| 6 | RUSSO, Liam | SO | DeSmet Jesuit High School | 43.04 | Verified |
| 7 | GRIFFIN, Colin | SO | DeSmet Jesuit High School | 43.87 | Verified |