| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROCHE, Andrew | JR | Vianney High School | NT | Unverified: |
| 2 | BAUR, Jeffrey | SR | St. Louis University High School | 57.14 | Verified |
| 3 | MOORE, Matthew | SO | St. Louis University High School | 52.78 | Verified |
| 4 | DUBOSE, Doreon | JR | Christian Brothers College High | 50.02 | Verified |
| 5 | THOMAS, Elijah | SR | DeSmet Jesuit High School | 52.68 | Verified |
| 6 | LEICHT, Jude | FR | Christian Brothers College High | 53.35 | Verified |
| 7 | LINDSEY, Jacob | SO | Vianney High School | 1:00.98 | Verified |