| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | Culleton | Battle | Pullam | Ivory | 46.11 | Verified |
| 3 | DeSmet Jesuit High School | A | Byas | Byerley | Hatcher | Pool | DQ | Verified |
| 4 | St. Louis University High School | A | Harris | Bailey | Chaffee | Wingo | 42.13 | Verified |
| 5 | Christian Brothers College High | A | Van | Hendricks | McClellan | Tillman | 43.19 | Verified |
| 6 | Chaminade College Preparatory Sc | A | Chri Jenkins | Collins | Taylor | Tim) Bailey | 44.97 | Verified |