| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis University High School | A | Kreuter | Lezieanya | Pace | Phoenix | 48.13 | Unverified: |
| 3 | DeSmet Jesuit High School | A | Stewart | Ramsey | King | McCaster | DQ | Unverified: |
| 4 | Christian Brothers College High | A | Morris | Hendrix | Cothrine | Williams | 45.59 | Unverified: |
| 5 | Vianney High School | A | Slade | Gamlin | Beckermann | Engelbart | 46.11 | Verified |
| 6 | Chaminade College Preparatory Sc | A | Brown | Dyson | Love | Kai) Robinson | 47.85 | Unverified: Lindbergh Invitational |