| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Elsberry Middle School | A | Rios | McNelly | Watts | Lovelace | 2:26.34 | Verified |
| 3 | Clopton Middle School | A | Frichett | Waller | Rickey | Liley | 1:58.40 | Verified |
| 4 | St. Louis Blue Knights Middle Sc | A | Adkisson | Boeckman | Ralston | Krestel | 2:13.07 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Callaway MS | A | Miller | Shirley | Wimmer | Miller | 1:53.75 | Verified |
| 2 | Mark Twain MS | A | Oglesley | Foss | Mixon | Bryan | 1:50.78 | Verified |
| 3 | Missouri Military Academy | A | De La Garza | Lowrey | Allen | Laszlo | 1:40.94 | Verified |
| 4 | Louisiana Middle School | A | Griffith | Miller | Settles | Ries | 1:49.36 | Verified |
| 5 | Winfield MS | A | Mense | Shinn | Trempler | Gebert | 1:53.53 | Verified |
| 6 | Griggsville-Perry Middle School | A | Rodgers | Brown | Kurfman | Booker | 1:56.18 | Verified |