| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis Blue Knights Middle Sc | A | Skogen | Drago | Owens | Riggs | 2:25.16 | Verified |
| 3 | Van-Far MS | A | Foster | Dameron | Griffith | Smith | 2:09.23 | Verified |
| 4 | Winfield MS | A | Amos | VanHook | Willenbrink | Johnson | 2:10.40 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain MS | A | Quinlin | Thompson | McKinney | Melvin | 2:07.96 | Verified |
| 2 | North Callaway MS | A | Kuda | Schmidt | Gordon | Wilson | 2:03.30 | Verified |
| 3 | Louisiana Middle School | A | Hutt | Chatman | Chatman | Griffith | 1:57.22 | Verified |
| 4 | Silex Middle School | A | Freeman | Morris | Harris | Harrell | 1:59.55 | Verified |
| 5 | Clopton Middle School | A | Wilson | Talbert | Stanek | Caplin | 2:05.86 | Verified |