| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 6 | St. Louis Blue Knights Middle Sc | A | Skogen | Stiles | Nicholls | Campbell | 11:20.43 | Verified |
| 7 | North Callaway MS | A | Vermette | Shryock | Boulware | Hartley | 11:50.71 | Verified |
| 8 | Winfield MS | A | Powers | Creamer | DuBois | Willenbrink | NT | Verified |
| 9 | Silex Middle School | A | Claborn | Lickteig | Harmon | Schwendemann | 12:09.24 | Verified |
| 10 | Louisiana Middle School | A | Thomas | Miller | Powell | Mooney | 12:27.48 | Verified |
| 11 | Clopton Middle School | A | VanBebber | Jensen | Talbert | Weaver | 12:54.47 | Verified |