| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wellsville-Middletown Middle Sch | A | Poage | Wilkinson | Joles | Nichols | 4:56.54 | Verified |
| 3 | Winfield MS | A | Stokes | Mense | Sandholm | Wilson | 4:46.82 | Verified |
| 4 | Clopton Middle School | A | Colbert | Lindsay | Malloy | Frichett | 4:49.56 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Silex Middle School | A | Heffner | Twellman | Freeman | Hallemeyer | 4:43.95 | Verified |
| 2 | St. Louis Blue Knights Middle Sc | A | Hagen | Muratori | Satterfield | Lily | 4:35.04 | Verified |
| 3 | Missouri Military Academy | A | Allen | Garritson | Jones | Clingenpeel | 4:04.80 | Verified |
| 4 | Van-Far MS | A | Hopke | Lynn | Puls | Stanich | 4:33.07 | Verified |
| 5 | Elsberry Middle School | A | Ford | Montes | Moore | Santos | 4:37.02 | Verified |
| 6 | Mark Twain MS | A | Baxter | Bogue | Branden | Wise | 4:43.99 | Verified |