| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Van-Far MS | A | Foster | Smith | Zumwalt | Keller | 5:23.67 | Verified |
| 3 | Mark Twain MS | A | Romig | Snodgrass | Redman | McKinney | 5:19.34 | Verified |
| 4 | Winfield MS | A | Creamer | Powers | VanHook | Willenbrink | 5:21.74 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Jensen | Martin | Wilson | Beauchamp | 5:11.15 | Verified |
| 2 | North Callaway MS | A | Hartley | Boulware | Sapp | Shryock | 5:07.71 | Verified |
| 3 | St. Louis Blue Knights Middle Sc | A | Criswell | Plymale | Nicholls | Clark | 4:46.68 | Verified |
| 4 | Louisiana Middle School | A | Miller | Griffin | Powell | Hutt | 5:04.44 | Verified |
| 5 | Silex Middle School | A | Claborn | Harmon | Lickteig | Schwendemann | 5:08.39 | Verified |