| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Louis Blue Knights Middle Sc | A | Ward | Clipper | Jacobs | Miller | DQ | Unverified: |
| 2 | Clopton Middle School | A | Frichett | Waller | Rickey | Liley | 57.85 | Verified |
| 3 | Silex Middle School | A | Hallemeyer | Heffner | Vehige | Mehler | 56.77 | Verified |
| 4 | Elsberry Middle School | A | Seely | Kinion | Watts | McNelly | 57.81 | Verified |
| 5 | Griggsville-Perry Middle School | A | Bradshaw | Dunham | Musgrave | Stover | 1:07.77 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain MS | A | Baxter | Bogue | Cruz | Bailey | 55.36 | Verified |
| 2 | Winfield MS | A | Galmiche | Gebert | Phelps | Mense | 51.66 | Verified |
| 3 | Missouri Military Academy | A | De La Garza | Lowrey | Laughlin | Allen | NT | Verified |
| 4 | Louisiana Middle School | A | McDaniel | Gschwender | Settles | Griffith | 49.97 | Verified |
| 5 | Van-Far MS | A | Wright-McAfee | Puls | Rhodes | Little | 54.26 | Verified |
| 6 | North Callaway MS | A | Matteson | Lobb | Horstman | Wimmer | 56.68 | Verified |