| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Griggsville-Perry Middle School | A | Kurfman | Kunzeman | Sheley | Miller | 1:04.32 | Verified |
| 3 | Van-Far MS | A | Carmen | Fraction | Griffith | Keller | 1:00.21 | Verified |
| 4 | Elsberry Middle School | A | Brinkman | Smith | Taylor | Wehmeyer | 1:01.32 | Verified |
| 5 | St. Louis Blue Knights Middle Sc | A | Miller | Nguyen | Owens | Majors | 1:15.55 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Silex Middle School | A | Freeman | Hutson | Klein | Harrell | 59.06 | Verified |
| 2 | North Callaway MS | A | Kuda | Atterberry | Schmidt | Gordon | 58.58 | Verified |
| 3 | Louisiana Middle School | A | Hutt | Chatman | Chatman | Warren | 54.82 | Verified |
| 4 | Mark Twain MS | A | Melvin | McKinney | Thompson | Moss | 55.90 | Verified |
| 5 | Winfield MS | A | Amos | Bray | Springer | Ohlms | 58.60 | Verified |
| 6 | Clopton Middle School | A | Weaver | Wilson | Stanek | Caplin | 59.25 | Verified |