| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Griggsville-Perry Middle School | A | Rodgers | Anstedt | Kurfman | Booker | NT | Unverified: |
| 3 | Clopton Middle School | A | Frichett | Waller | Liley | Rickey | 2:01.31 | Verified |
| 4 | Elsberry Middle School | A | Moore | McNelly | Seely | Wright | DQ | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City Middle School | A | Meyer | Shinn | Heaston | Reyes | 1:53.00 | Verified |
| 2 | Palmyra MS | A | Harper | Kroeger | Snow | Gibson | 1:51.96 | Verified |
| 3 | Bowling Green Middle School | A | Berry | Huckstep | Welch | Perkins | 1:48.31 | Verified |
| 4 | Louisiana Middle School | A | Ries | Ries | Miller | Settles | 1:51.13 | Verified |
| 5 | Mark Twain MS | A | Bryan | Mixon | Foss | Oglesley | 1:52.00 | Verified |
| 6 | Winfield MS | A | Shinn | Mense | Keeteman | Gebert | 1:55.24 | Verified |