| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Elsberry Middle School | A | Burbridge | Wehmeyer | Baker | Robbins | NT | Unverified: |
| 2 | Holy Rosary Middle School | A | Fuemmeler | Gares | Thompson | Williams | 2:14.54 | Verified |
| 3 | Clopton Middle School | A | Wilson | Talbert | Stanek | Caplin | 2:12.30 | Verified |
| 4 | Van-Far MS | A | Griffith | Dameron | Keller | Carmen | 2:13.69 | Verified |
| 5 | North Callaway MS | A | Kuda | Schmidt | Wilson | Bears | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain MS | A | Quinlin | McKinney | Romig | Melvin | 2:07.96 | Verified |
| 2 | Palmyra MS | A | Hudson | Moore | James | Plunkett | 2:04.55 | Verified |
| 3 | Silex Middle School | A | Harrell | Hutson | Kuntz | Lickteig | 2:04.08 | Verified |
| 4 | Bowling Green Middle School | A | Eskew | Grote | Grote | Hustedde | 2:04.16 | Verified |
| 5 | Louisiana Middle School | A | Hutt | Chatman | Chatman | Reed | 2:07.32 | Verified |
| 6 | Winfield MS | A | Amos | Powers | Springer | VanHook | 2:10.40 | Verified |