| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Winfield MS | A | Wilson | Stark | Mense | Stokes | 5:03.06 | Verified |
| 3 | Clopton Middle School | A | Palmer | Frichett | Lindsay | Simmons | 4:49.82 | Verified |
| 4 | Mark Twain MS | A | Baxter | Bogue | Branden | Wise | 4:54.64 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Van-Far MS | A | Keller | Stanich | Puls | Hopke | 4:45.40 | Verified |
| 2 | Monroe City Middle School | A | McNeill | Reyes | Lehenbauer | Shinn | 4:31.04 | Verified |
| 3 | Bowling Green Middle School | A | Bair | Hickerson | McDannold | Welch | 4:16.39 | Verified |
| 4 | Palmyra MS | A | McNeff | Kroeger | Schlueter | Gibson | 4:16.73 | Verified |
| 5 | Silex Middle School | A | Twellman | Mehler | Hallemeyer | Heffner | 4:43.95 | Verified |