| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Van-Far MS | A | Zumwalt | Utterback | Foster | Smith | NT | Unverified: |
| 2 | Winfield MS | A | Santonge | Powers | VanHook | Bray | 5:51.42 | Verified |
| 3 | Mark Twain MS | A | Romig | Snodgrass | Redman | McKinney | 5:30.05 | Verified |
| 4 | Monroe City Middle School | A | Quinn | Hermann | Richardson | Russell | 5:36.78 | Verified |
| 5 | North Callaway MS | A | Shryock | Hartley | Boulware | Sapp | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Holy Rosary Middle School | A | Lincoln | Lucas | Gares | Hays | 5:18.18 | Verified |
| 2 | Silex Middle School | A | Schwendemann | Lickteig | Harmon | Klein | 5:13.90 | Verified |
| 3 | Bowling Green Middle School | A | Vanhooser | Grote | Hustedde | Barnes | 4:54.28 | Verified |
| 4 | Palmyra MS | A | James | Pontius | Meyer | Timbrook | 4:59.42 | Verified |
| 5 | Louisiana Middle School | A | Powell | Griffin | Welch | Miller | 5:17.46 | Verified |
| 6 | Clopton Middle School | A | Weaver | Wilson | Beauchamp | Jensen | 5:25.33 | Verified |