| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Griggsville-Perry Middle School | A | Bradshaw | Dunham | Musgrave | Stover | NT | Unverified: |
| 2 | Clopton Middle School | A | Frichett | Waller | Rickey | Liley | 58.47 | Verified |
| 3 | Van-Far MS | A | Puls | Martin | Lynn | Stanich | 57.63 | Verified |
| 4 | Silex Middle School | A | Heffner | Mehler | Van Horn | Hallemeyer | 57.83 | Verified |
| 5 | Wellsville-Middletown Middle Sch | A | Farr | Jones | Pike | Freie | 1:13.82 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain MS | A | Cruz | Bogue | Lafontaine | Bailey | 55.58 | Verified |
| 2 | Palmyra MS | A | Harper | Snow | Schlueter | Gibson | 52.87 | Verified |
| 3 | Louisiana Middle School | A | Gschwender | Miller | Settles | Griffith | 50.76 | Verified |
| 4 | Winfield MS | A | Phelps | Keeteman | Galmiche | Gebert | 51.66 | Verified |
| 5 | Monroe City Middle School | A | Nash | Heaston | Meyer | Reyes | 54.53 | Verified |
| 6 | Bowling Green Middle School | A | Benn | Smith | Vodohosky | Potter | 57.09 | Verified |