| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Griggsville-Perry Middle School | A | Kurfman | Kunzeman | Sheley | Miller | NT | Unverified: |
| 3 | Wellsville-Middletown Middle Sch | A | Brennan | Reese | Geisler | Harmon | 1:11.84 | Verified |
| 4 | North Callaway MS | A | Kuda | Atterberry | Schmidt | Wilson | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Wilson | Weaver | Stanek | Caplin | 1:04.65 | Verified |
| 2 | Elsberry Middle School | A | Brinkman | Wehmeyer | Taylor | Smith | 1:01.32 | Verified |
| 3 | Van-Far MS | A | Jones | Carmen | Fraction | Keller | 1:00.21 | Verified |
| 4 | Holy Rosary Middle School | A | Williams | Willingham | Mulvaney | Lincoln | 1:00.39 | Verified |
| 5 | Pleasant Hill MS | A | Hubbard | Al-Rawas | Johnson | Highins | 1:03.27 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Silex Middle School | A | Kuntz | Harrell | Hutson | Freeman | 59.06 | Verified |
| 2 | Palmyra MS | A | Anderson | James | Martin | Meyer | 57.61 | Verified |
| 3 | Louisiana Middle School | A | Griffith | Reed | Chatman | Chatman | 56.04 | Verified |
| 4 | Mark Twain MS | A | Melvin | McKinney | Gilbert | Moss | 56.28 | Verified |
| 5 | Winfield MS | A | Amos | VanCleve | Bray | Ohlms | 58.96 | Verified |
| 6 | Bowling Green Middle School | A | Grote | Akers | Barnes | Hustedde | 59.31 | Verified |