| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ELLIS, Matera | SR | Mark Twain High School | 6:01.00 | Verified |
| 2 | BRANDT, Sadie | SR | St. Louis Blue Knights | 6:13.29 | Verified |
| 3 | STAHL, Kaycie | JR | Highland High School | 6:14.81 | Verified |
| 4 | CLABORN, MCKENNA | SO | Silex High School | 6:18.59 | Verified |
| 5 | BIBB, Dianna | SO | Bowling Green High School | 6:32.51 | Verified |
| 6 | STILES, Anya | SO | St. Louis Blue Knights | 6:38.89 | Verified |
| 7 | LILLEY, Addison | FR | Bowling Green High School | 6:42.13 | Verified |
| 8 | CHILDS, Erin | SO | Van-Far High School | 6:59.72 | Verified |
| 9 | KINION, CLAIRE | SO | Silex High School | 7:11.50 | Verified |
| 10 | GRESHAM, Abi | JR | St. Louis Blue Knights | 7:31.06 | Verified |
| 11 | HARKE, Gracin | FR | Silex High School | NT | Unverified: |