| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton High School | A | Burt | Adams | West | Harvey | DNS | Verified |
| 3 | Missouri Military Academy | A | Carter | Elford | McBride | Stallone | 1:40.94 | Verified |
| 4 | Wellsville-Middletown High Schoo | A | Henderson | Huff | Mandrell | Vogel | 1:43.05 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Van-Far High School | A | Baugh | Selsor | Hartley | Gaston | 1:40.91 | Verified |
| 2 | Bowling Green High School | A | Oberhaus | Grote | Ream | Niemeyer | 1:39.00 | Verified |
| 3 | Highland High School | A | Stegeman | Gaus | Stutsman | Hinton | 1:35.67 | Verified |
| 4 | Louisiana High School | A | Mayfield | Dzierwa | Luck | Greene | 1:36.00 | Verified |
| 5 | Mark Twain High School | A | Tobin | Zumwalt | Bennett | Bergthold | 1:39.09 | Verified |
| 6 | St. Louis Blue Knights | A | Mudge | Leine | Seth | Bryant | 1:40.92 | Verified |