| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wellsville-Middletown High Schoo | A | Boeckelman | Boeckelman | Scott | Stroup | 2:10.68 | Verified |
| 3 | St. Louis Blue Knights | A | Nguyen | Plymale | Knoerr | Plymale | 2:08.95 | Verified |
| 4 | Clopton High School | A | Palmer | Myers | Simmons | Bankhead | 2:10.61 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bowling Green High School | A | Berry | Dean | Dawson | Korte | 2:02.19 | Verified |
| 2 | Louisiana High School | A | Campbell | Ries | Chatman | Pedersen | 1:54.47 | Verified |
| 3 | Highland High School | A | Bringer | Keith | Abell | Reed | 1:53.39 | Verified |
| 4 | Silex High School | A | Thoroughman | Hall | Van Horn | Weatherford | 1:54.15 | Verified |
| 5 | Mark Twain High School | A | Boleach | Hudelson | Dotson | Ross | DNS | Verified |
| 6 | Van-Far High School | A | Brooksher | Caldwell | Shelton | Skwirtz | 2:08.90 | Verified |