| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Missouri Military Academy | A | Paredes | Salinas | Powell | Miles | 4:04.80 | Verified |
| 2 | St. Louis Blue Knights | A | Schark | Leine | Seth | Bryant | 3:46.79 | Verified |
| 3 | Mark Twain High School | A | Preston | Black | Northcutt | McLeod | 3:37.67 | Verified |
| 4 | Bowling Green High School | A | Hembree | Ream | Matlock | Finnegan | 3:45.07 | Verified |
| 5 | Highland High School | A | Oening | Hinton | Evans | Stegeman | 3:56.19 | Verified |
| 6 | Silex High School | A | Brennan | Carroll | Noxon | Noxon | DNS | Unverified: |