| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Silex High School | A | Pickens | Klein | Kuntz | Isom | 4:46.97 | Verified |
| 2 | Louisiana High School | A | Lentz | Campbell | Bowser | Pedersen | 4:40.17 | Verified |
| 3 | Highland High School | A | Reed | Keith | Abell | Sharpe | 4:24.20 | Verified |
| 4 | St. Louis Blue Knights | A | Bryant | Plymale | Plymale | Brown | 4:28.88 | Verified |
| 5 | Bowling Green High School | A | Berry | Turner | Korte | Lilley | 5:03.11 | Verified |
| 6 | Mark Twain High School | A | Miller | Hudelson | Dotson | Norton | 4:41.36 | Verified |