| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | McLeod | Black | Miller | Northcutt | 8:42.36 | Verified |
| 2 | Missouri Military Academy | A | Grantz | Pehle | Pfeifer | Villarreal | 9:23.12 | Verified |
| 3 | Highland High School | A | Self | Evans | Caudill | Clow | 9:44.57 | Verified |
| 4 | Wellsville-Middletown High Schoo | A | Bickell | Marshall | Slovensky | Vogel | 9:50.73 | Verified |
| 5 | St. Louis Blue Knights | A | Schellman | Anderson | Meyers | Ward | 10:00.01 | Verified |