| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ABELL, Addy | FR | Highland High School | NT | Unverified: |
| 3 | SIMMONS, Sophie | FR | Clopton High School | 33.56 | Verified |
| 4 | KNOERR, Grace | SO | St. Louis Blue Knights | NT | Unverified: |
| 5 | BROWN, Faith | FR | St. Louis Blue Knights | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CALDWELL, Madelynn | SR | Van-Far High School | 32.17 | Verified |
| 2 | LESLEY, Michela | FR | Louisiana High School | 30.76 | Verified |
| 3 | TURNER, Reese | FR | Bowling Green High School | 29.75 | Unverified: |
| 4 | ROSS, Audrey | SR | Mark Twain High School | 29.87 | Verified |
| 5 | BOECKELMAN, Natalie | SR | Wellsville-Middletown High Schoo | 31.60 | Verified |
| 6 | MAGGARD, Mariah | FR | Van-Far High School | 32.85 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RIES, Jalaya | SO | Louisiana High School | 29.57 | Verified |
| 2 | ORTEGA, Starlit | SO | Bowling Green High School | 29.30 | Verified |
| 3 | VAN HORN, Austyn | FR | Silex High School | 29.23 | Verified |
| 4 | TALBERT, Stacia | JR | Clopton High School | 29.30 | Verified |
| 5 | HAWN, Kadence | SR | Mark Twain High School | 29.40 | Verified |
| 6 | PLYMALE, Cami | FR | St. Louis Blue Knights | 29.69 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WEATHERFORD, Sarah | JR | Silex High School | 28.82 | Verified |
| 2 | PEDERSEN, Jordan | SO | Louisiana High School | 28.01 | Verified |
| 3 | BOLEACH, Madison | JR | Mark Twain High School | 27.46 | Verified |
| 4 | SLOVENSKY, Bethany | JR | Wellsville-Middletown High Schoo | 27.91 | Verified |
| 5 | HALL, Maddy | SR | Silex High School | 28.36 | Verified |
| 6 | TASCO, Amelia | SR | Highland High School | 29.17 | Verified |