| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRYANT, Tabitha | SO | St. Louis Blue Knights | 2:36.74 | Verified |
| 2 | MILLER, Sydney | SO | Mark Twain High School | 2:43.16 | Verified |
| 3 | FORMAN, Brooklyn | JR | Van-Far High School | 2:45.99 | Verified |
| 4 | BRANDT, Sadie | SR | St. Louis Blue Knights | 2:51.04 | Verified |
| 5 | ISOM, Maddison | JR | Silex High School | 2:51.07 | Verified |
| 6 | BIBB, Dianna | SO | Bowling Green High School | 2:57.71 | Verified |
| 7 | AUER, Audrey | FR | Silex High School | 2:59.88 | Verified |
| 8 | ROBERTS, Ava | FR | Mark Twain High School | 3:00.15 | Verified |
| 9 | TREDWAY, Ashlynn | JR | Louisiana High School | 3:00.71 | Verified |
| 10 | HARKE, Gracin | FR | Silex High School | 3:01.20 | Verified |
| 11 | TRUETKEN, Katherine | FR | Clopton High School | 3:05.90 | Verified |
| 12 | EGGEMEYER, Victoria | JR | Clopton High School | 3:16.85 | Verified |
| 13 | SPERRY, Kendra | SO | Louisiana High School | NT | Unverified: |
| 14 | WELLS, Autumn | FR | Bowling Green High School | NT | Unverified: |
| 15 | STILES, Anya | SO | St. Louis Blue Knights | NT | Unverified: |