| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CHANDLER, Haiden | FR | Bowling Green High School | NT | Unverified: |
| 3 | NGUYEN, Sophia | SO | St. Louis Blue Knights | 1:02.81 | Verified |
| 4 | HICKS, Sophie | FR | Mark Twain High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAJORS, Emily | SO | St. Louis Blue Knights | 59.90 | Verified |
| 2 | NORVELL, Harley | JR | Louisiana High School | 58.27 | Verified |
| 3 | BANKHEAD, Ava | FR | Clopton High School | 57.46 | Verified |
| 4 | CHARLTON, Sydney | SO | Bowling Green High School | 57.97 | Verified |
| 5 | GRESHAM, Hannah | JR | St. Louis Blue Knights | 58.96 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JAMES, Ashley | SO | Silex High School | 56.72 | Verified |
| 2 | KINION, CLAIRE | SO | Silex High School | 56.39 | Verified |
| 3 | THOROUGHMAN, Rita | SR | Silex High School | 49.09 | Verified |
| 4 | BRINGER, Ansley | SR | Highland High School | 50.78 | Verified |
| 5 | MYERS, Faith | SR | Clopton High School | 56.58 | Verified |
| 6 | CAPLIN, Brittany | FR | Clopton High School | 57.36 | Unverified: |