| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MAYNARD, Alexea | FR | Bowling Green High School | 1:28.09 | Verified |
| 3 | SIMMONS, Sophie | FR | Clopton High School | 1:19.64 | Verified |
| 4 | HAYS, Addyson | FR | Van-Far High School | 1:23.17 | Verified |
| 5 | MAJORS, Emily | SO | St. Louis Blue Knights | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MITCHELL, Avenley | FR | Clopton High School | 1:14.74 | Verified |
| 2 | CALDWELL, Madelynn | SR | Van-Far High School | 1:12.22 | Verified |
| 3 | DOTSON, Riley | FR | Mark Twain High School | 1:11.71 | Verified |
| 4 | NORTON, Lucille | FR | Mark Twain High School | 1:11.95 | Verified |
| 5 | NGUYEN, Sophia | SO | St. Louis Blue Knights | 1:13.75 | Verified |
| 6 | ETCHER, Sara | JR | St. Louis Blue Knights | 1:17.56 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ISOM, Maddison | JR | Silex High School | 1:10.50 | Verified |
| 2 | KLEIN, Sydney | 8 | Silex High School | 1:07.11 | Verified |
| 3 | SHARPE, Chevie | FR | Highland High School | 1:00.36 | Verified |
| 4 | SLOVENSKY, Bethany | JR | Wellsville-Middletown High Schoo | 1:04.00 | Verified |
| 5 | TALBERT, Stacia | JR | Clopton High School | 1:07.51 | Verified |
| 6 | PICKENS, Emma | SR | Silex High School | 1:11.64 | Verified |