| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton High School | A | Burt | Adams | West | Harvey | DNS | Unverified: |
| 3 | St. Louis Blue Knights | A | Schark | Ward | Gaugh | Bryant | 49.36 | Verified |
| 4 | Van-Far High School | A | Evans | Gaston | Sharp | Baugh | 50.86 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Tobin | Bennett | McLeod | Preston | 48.09 | Verified |
| 2 | Missouri Military Academy | A | Kahle | Salinas | Erskine | Burgett | DQ | Verified |
| 3 | Bowling Green High School | A | Niemeyer | Ream | Buffington | Lewis | 44.58 | Verified |
| 4 | Louisiana High School | A | Blackwell | Dzierwa | Luck | Greene | 45.52 | Verified |
| 5 | Highland High School | A | Stegeman | Hinton | Moore | Gaus | 47.59 | Verified |