| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis Blue Knights | A | Etcher | Knoerr | Riggs | Gresham | 1:02.22 | Verified |
| 3 | Van-Far High School | A | Brooksher | Jensen | Maggard | 1:00.67 | Verified | |
| 4 | Wellsville-Middletown High Schoo | A | Boeckelman | Boeckelman | Scott | Stroup | 1:00.92 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Boleach | Hudelson | Hawn | Ross | 55.24 | Verified |
| 2 | Louisiana High School | A | Campbell | Ries | Chatman | Wallace | 53.81 | Verified |
| 3 | Bowling Green High School | A | Gamm | Dawson | Turner | Johnson | 53.55 | Verified |
| 4 | Silex High School | A | Thoroughman | Hall | Kuntz | Weatherford | 53.67 | Verified |
| 5 | Highland High School | A | Bringer | Keith | Reed | Sharpe | 53.85 | Verified |
| 6 | Clopton High School | A | Bankhead | Palmer | Talbert | Williamson | 59.44 | Verified |